Francoy, T.M., Grassi M.L., Imperatriz-Fonseca V.L., May-Itzá W. de J., Quezada-Euán, J.J.G. 2011. Geometric morphometrics of the wing as a tool for assigning genetic lineages and geographic origin in Melipona beecheii (Hymenoptera:Meliponini). Apidologie 42:499–507.
Abstract
The stingless bee Melipona beecheii
presents great variability and is considered a complex of species. In
order to better understand this species complex, we need to evaluate its
diversity and develop methods that allow geographic traceability of the
populations. Here we present a fast, efficient, and inexpensive means
to accomplish this using geometric morphometrics of wings. We collected
samples from Mexico, Guatemala, El Salvador, Nicaragua, and Costa Rica
and we were able to correctly assign 87.1% of the colonies to their
sampling sites and 92.4% to their haplotype. We propose that geometric
morphometrics of the wing could be used as a first step analysis leaving
the more expensive molecular analysis only to doubtful cases.
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